{"created":"2023-05-15T12:52:15.865424+00:00","id":1722,"links":{},"metadata":{"_buckets":{"deposit":"e1d8033c-0df0-47d8-97d7-b7e10bf9f6d8"},"_deposit":{"created_by":14,"id":"1722","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"1722"},"status":"published"},"_oai":{"id":"oai:omnh.repo.nii.ac.jp:00001722","sets":["1:294"]},"author_link":["4602","4598","4601","4600","4599"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"77","bibliographicPageEnd":"36","bibliographicPageStart":"29","bibliographic_titles":[{"bibliographic_title":"大阪市立自然史博物館研究報告"},{"bibliographic_title":"Bulletin of the Osaka Museum of Natural History","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"博物館の乾燥菌類標本のカビ汚染について調査を行ったところ，カビはDNAとして検出されるが，現在生存していないことが明らかになった．そこで，過去に採集，乾燥，標本の作製，保存のどの段階で，どの程度カビ汚染が起きたかについて，博物館に収蔵されているAmanita属36標本のカサの部分を用いて検証した．手法としては，２種のカビの作成したプライマーを用いて，各検体のカビ数をリアルタイムPCRで測定し，その胞子数を調査した．得られた結果は次の通りであった．①両種の胞子数は標本ごとに桁違いのバラツキがあった．②検出されたA. penicillioidesの胞子数は，標本の乾重量100 mg当たり平均約1000個, Eurotium sp. は約7個であった．③結果は，採集時からの様々な過程で，湿り気が発生し，標本上にカビが一時的に発生したことを示唆している．なお，産出された胞子数は標本の古さ，あるいは目視でのカビの有無とは関係ないので，保存前の段階で汚染がしばしば起きたと推測される．","subitem_description_type":"Abstract"},{"subitem_description":"Fungal contamination on dried mushroom specimens in the museum were tested. Alive fungal spores were not found in specimens examined, but DNA of dead fungal spores was often found in them. We elucidated when this fungal contamination had occurred. Spores of dead common xerophilic fungi, Aspergillus penicillioides and Eurotium sp. in each dry specimen of Amanita was measured by real-time PCR. Trace of fungal contamination was found in many specimens examined. A large variation of spore counts was recognized among specimens in logarithmic scale, and A. penicillioides was predominant. Thus, the propagation of fungi was thought to occur after collecting specimen, for example drying sample of mushrooms, and storage of specimens. Because fungal contamination occurred regardless stored period of specimens in the museum, a lot of this contamination in specimens seems to occur before storage in the museum according to dampness in surrounding environments. ","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20643/00001671","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"大阪市立自然史博物館"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0078-6675","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"浜田, 信夫"},{"creatorName":"ハマダ, ノブオ","creatorNameLang":"ja-Kana"},{"creatorName":"HAMADA, Nobuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4598","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田口, 淳二"},{"creatorName":"タグチ, ジュンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4599","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐久間, 大輔"},{"creatorName":"サクマ, ダイスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4600","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TAGUCHI, Junji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4601","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SAKUMA, Daisuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4602","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-31"}],"displaytype":"detail","filename":"03_Hamada_29-36.pdf","filesize":[{"value":"970.8 kB"}],"format":"application/pdf","licensetype":"license_9","mimetype":"application/pdf","url":{"label":"03_Hamada_29-36","url":"https://omnh.repo.nii.ac.jp/record/1722/files/03_Hamada_29-36.pdf"},"version_id":"3afbb7cc-d57a-43b7-80c2-bcbf4da8625f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aspergillus penicillioides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"conservation science","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"natural history specimen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"herbarium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mushroom collection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"real-time PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"博物館所蔵菌類標本へのカビ汚染についてのリアルタイムPCRによる追跡","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"博物館所蔵菌類標本へのカビ汚染についてのリアルタイムPCRによる追跡"},{"subitem_title":"Trace of fungal contamination on specimens of mushrooms stored in museum by real-time PCR","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"14","path":["294"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-03-31"},"publish_date":"2023-03-31","publish_status":"0","recid":"1722","relation_version_is_last":true,"title":["博物館所蔵菌類標本へのカビ汚染についてのリアルタイムPCRによる追跡"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-05-15T12:55:34.205565+00:00"}